The reaction was quenched by addition of 2 mL saturated sodium bicarbonate, extracted 2 10 mL EtOAc, washed with brine, dried over sodium sulfate, filtered and concentrated in vacuo, purified on silica gel, 0 100 3 1 EtOAc EtOH buy propecia Second, the high integration frequency of SB100X transposase and the strong CAG promoter guarantee high expression efficiency of target genes