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    The reaction was quenched by addition of 2 mL saturated sodium bicarbonate, extracted 2 10 mL EtOAc, washed with brine, dried over sodium sulfate, filtered and concentrated in vacuo, purified on silica gel, 0 100 3 1 EtOAc EtOH buy propecia Second, the high integration frequency of SB100X transposase and the strong CAG promoter guarantee high expression efficiency of target genes

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