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Hereditary mutations in the BMPR1A gene encoding the BMP type I receptor BMPRIA ALK 3 cause juvenile polyposis, an autosomal dominant genetic disorder that predisposes patients to intestinal polyposis and cancer MassaguГ© et al buy priligy online safe Here, we describe a stepwise protocol for the generation and characterization of such an iPSC line AAVS1 PDi Cas9 iPSC with a doxycycline dox inducible Cas9 expression cassette from the AAVS1 safe harbor site and efficient editing of target genes with lentiviral vectors expressing gRNAs